Data analysis were performed using two independent sample test formula by SPSS 20 for windows. The data of critical thinking abilities are tested in this study consist of 5 indicator skills: (1) formulate the problem (2) recognize the existence of a logical relationship between issues (3) collect and collate information (4) asses and evaluate the statement of facts (5) Make some conclude were needed. Field trial testing using a pre-test post-test control group design. The result of limited testing showed that each of percentage score average in biology practical guidance based on TGT and practical performance assessment instrument were 82,67% and 83,33% and categorized as “very good”. Limited testing conducted on 10 students with high, medium, and low ability. The result of the expert validation by 3 validators showed that each of the average score in biology practical guidance based on TGT and practical performance assessment instrument were 4,41 and 4,48 and categorized as “very good”. Product development process in this study by following Borg and Gall’s Development. Abstract The aims of this study were: 1) to develop a biology practical guidance based on TGT cooperative models equipped with practical performance assessment instrument 2) to determine the characteristics and suitability of products than have been developed 3) to know it’s effectiveness in critical thinking abilities on biology subject of students in class XI.
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